When you're restless, you find it hard to sit still or concentrate on anything. An audience often grows restless when they're waiting for the second act of a play to start and there's an unexpected delay.
If you think of restless as "unable to rest or relax," you'll know exactly what it means. You might have a restless night if you can't fall asleep and spend hours tossing and turning. A bunch of elementary school kids listening to a boring speech will grow restless in a way that's hard to miss, since they're bound to be squirming in their seats.